We are excited to share our latest preprint on bioRxiv exploring how oocytes build and organize the nucleolus to support the extraordinary demands of egg development. Using zebrafish and mouse oocytes, we uncover extensive remodeling of nucleolar architecture, reveal how amplified ribosomal DNA is organized within the nucleus, and show how nucleolar structure and function change throughout oogenesis. This work was made possible through a wonderful collaboration with the Gerton lab and the support of many outstanding Stowers technology centers, including Aquatics, Microscopy, and Histology. We are grateful to everyone who contributed to this project and look forward to sharing it with the broader scientific community.